产品名称
Human FSTL1 ELISA Kit
指标别名
follistatin like 1; Follistatin like protein 1; Follistatin related protein 1; FRP; FSL1; FSTL1; TSC-36
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
312 pg/ml - 20000 pg/ml
文献引用格式
Human FSTL1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0965)
背景资料
Follistatin-related protein 1, also called FSTL1 or FRP is a protein that in humans is encoded by the FSTL1 gene. FSTL1 gene is mapped to chromosome 3q13.33 based on an alignment of FSTL1 sequence with the genomic sequence(GRCh37). This gene encodes a protein with similarity to follistatin, an activin-binding protein. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. This gene product is thought to be an autoantigen associated with rheumatoid arthritis. This gene may modulate the action of some growth factors on cell proliferation and differentiation. It was suggested that fibroblast-secreted FSTL1 acts on macrophages to increase TNF and IL1B production and IL6 activity.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0965-CAP | 96-well plate precoated with anti- human FSTL1 antibody | 1 |
EK0965-ST | Lyophilized recombinant human FSTL1 standard | 20ng/tube×2 |
EK0965-DA | Biotinylated anti- human FSTL1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 591 | 1511 | 9939 | 616 | 1368 | 9532 |
Standard deviation | 26.59 | 111.81 | 397.56 | 38.8 | 106.7 | 390.81 |
CV(%) | 4.5 | 7.4 | 4 | 6.3 | 7.8 | 4.1 |
典型数据示例
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.101 | 0.156 | 0.198 | 0.337 | 0.606 | 1.071 | 1.770 | 2.333 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)