产品名称
Rat TGF Beta 3/TGFB3 ELISA Kit
指标别名
ARVD; TGF beta 3; TGF beta3; TGFB3; TGF-beta 3; TGF-β3
免疫原
Expression system for standard: NS0; Immunogen sequence: A301-S412
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
transforming growth factor, beta 3
文献引用格式
Rat TGF Beta 3/TGFB3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1105)
背景资料
Transforming growth factor beta 3 (TGF-beta 3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily. TGF-beta 3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix (ECM) formation during the process of palate development. Without TGF-beta 3, mammals develop a deformity known as acleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-beta 3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin. TGF-beta 3 activated Lef1 in the absence of beta-catenin (CTNNB1) via nuclear phospho-Smad2 and Smad4.
试剂盒内容
Catalog number | Description | Quantity |
EK1105-CAP | 96-well plate precoated with anti- rat TGF-beta 3 antibody | 1 |
EK1105-ST | Lyophilized recombinant rat TGF-beta 3 standard | 10ng/tube×2 |
EK1105-DA | Biotinylated anti- rat TGF-beta 3 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 77 | 234 | 841 | 81 | 242 | 805 |
Standard deviation | 5.92 | 14.97 | 42.89 | 7.12 | 18.15 | 41.86 |
CV(%) | 7.7% | 6.4% | 5.1% | 8.8% | 7.5% | 5.2% |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.018 | 0.126 | 0.223 | 0.430 | 0.812 | 1.379 | 2.135 | 2.505 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (EDTA) and Urine