Human Azurocidin/AZU1 ELISA Kit

96T/2480

azurocidin 1; AZAMP; AZU; CAP37; HBP; HUMAZUR; NAZC; Hhbp; Azurocidin; AZU1

NSO, I27-P248

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

62.5 pg/ml - 4000 pg/ml

AZU1

azurocidin 1

P20160

AB_2936229

Human Azurocidin/AZU1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1161)

Azurocidin, also known as cationic antimicrobial protein CAP37 or heparin-binding protein (HBP), is a protein that in humans is encoded by the AZU1 gene. This encoded protein is a member of the serine protease gene family, but it is not a serine proteinase, because the active site serine and histidine residues are replaced. Azurocidin is mapped to 19p13.3. The protein encoded by this gene is an azurophil granule antibiotic protein, with antibacterial activity. It is also an important multifunctional inflammatory mediator. In addition to it, Azurocidin is also a specific chemoattractant for monocytes. It lacks the chemotactic activity for neutrophils and lymphocytes, and this gene is probably responsible for the wave of monocytes that follows the initial wave of PMNs typical of the inflammatory response.

20-25分钟

Catalog number	Description	Quantity
EK1161-CAP	96-well plate precoated with anti- human Azurocidin antibody	1
EK1161-ST	Lyophilized recombinant human Azurocidin standard	10ng/tube×2
EK1161-DA	Biotinylated anti- human Azurocidin antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	178	606	1031	175	567	1093
Standard deviation	9.79	39.99	75.26	9.62	38.55	96.18
CV(%)	5.5	6.6	7.3	5.5	6.8	8.8

Concentration(pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.015	0.046	0.070	0.121	0.293	0.650	1.514	2.260

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

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Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h