产品名称
Mouse ACE2 ELISA Kit
指标别名
ACE related carboxypeptidase; ACE2; ACEH; Angiotensin converting enzyme 2; Metalloprotease MPROT15
免疫原
Expression system for standard: CHO; Immunogen sequence: Q18-T740
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Angiotensin-converting enzyme 2
文献引用格式
Mouse ACE2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1188)
背景资料
AngiotensinI-converting enzyme 2 (ACE 2) is a protein belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. By sequence similarity to a sequence in GenBank, this gene is mapped to Xp22.2. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1188-CAP | 96-well plate precoated with anti- mouse ACE2 antibody | 1 |
EK1188-ST | Lyophilized recombinant mouse ACE2 standard | 10ng/tube×2 |
EK1188-DA | Biotinylated anti- mouse ACE2 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 146 | 538 | 1252 | 131 | 557 | 1192 |
Standard deviation | 11.38 | 25.28 | 76.37 | 10.48 | 31.74 | 84.63 |
CV(%) | 7.8% | 4.7% | 6.1% | 8% | 5.7% | 7.1% |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.055 | 0.091 | 0.132 | 0.226 | 0.412 | 0.730 | 1.331 | 2.160 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin)