产品名称
Human Adipsin/CFD ELISA Kit
指标别名
ADIPSIN; ADN; C3 convertase activator; CFD; Complement factor D; complement factor D (adipsin); DF; PFD; Properdin factor D
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
312 pg/ml - 20000 pg/ml
基因名全称
complement factor D (adipsin)
文献引用格式
Human Adipsin/CFD ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1199)
背景资料
Complement factor D(CFD), also called ADIPSIN or FACTOR D, is a protein which in humans is encoded by the CFD gene. The protein encoded by this gene belongs to the trypsin family of peptidases. It is mapped to 19p13.3. The encoded protein is a component of the alternative complement pathway which is best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. What's more, Factor D is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1199-CAP | 96-well plate precoated with anti- human CFD antibody | 1 |
EK1199-ST | Lyophilized recombinant human CFD standard | 20ng/tube×2 |
EK1199-DA | Biotinylated anti- human CFD antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 787 | 2378 | 9095 | 804 | 2193 | 8576 |
Standard deviation | 44.07 | 149.81 | 436.56 | 49.84 | 144.73 | 471.68 |
CV(%) | 5.6 | 6.3 | 4.8 | 6.2 | 6.6 | 5.5 |
典型数据示例
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.009 | 0.093 | 0.182 | 0.358 | 0.701 | 1.156 | 1.822 | 2.355 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Urine