产品名称
Mouse F11R ELISA Kit
指标别名
CD321; F11 receptor; F11R; JAM; JAM 1; JAM A; JAM1; JAMA; JAMA1; JCAM; Junctional adhesion molecule 1; Junctional adhesion molecule A; KAT; PAM 1; Platelet adhesion molecule 1; Platelet F11 receptor
免疫原
Expression system for standard: NS0; Immunogen sequence: K27-G238
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Junctional adhesion molecule A
文献引用格式
Mouse F11R ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1228)
背景资料
Junctional adhesion molecule A (JAM-A) is a protein that in humans is encoded by the F11R gene. It is mapped to 1q23.3. This gene is an immunoglobulin-like molecule that colocalizes with tight junctions in endothelium and epithelium and is also found on blood leukocytes and platelets. JAM-A plays an important role in the regulation of tight junction assembly in epithelia. In addition, it can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration, and a platelet receptor. JAM-A has a nonredundant role in controlling DC motility, trafficking to lymph nodes, and activation of specific immunity.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1228-CAP | 96-well plate precoated with anti- mouse JAM-A antibody | 1 |
| EK1228-ST | Lyophilized recombinant mouse JAM-A standard | 10ng/tube×2 |
| EK1228-DA | Biotinylated anti- mouse JAM-A antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 74 | 243 | 871 | 67 | 225 | 792 |
| Standard deviation | 3.18 | 18.95 | 57.48 | 3.28 | 18.67 | 61.77 |
| CV(%) | 4.3% | 7.8% | 6.6% | 4.9% | 8.3% | 7.8% |
典型数据示例
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.018 | 0.035 | 0.059 | 0.125 | 0.303 | 0.648 | 1.697 | 2.869 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)