产品名称
Mouse Angiopoietin-1/ANGPT1 ELISA Kit
指标别名
AGP1; AGPT; ANG 1; ANG1; angiopoietin 1; Angiopoietin-1; ANGPT1; KIAA0003
免疫原
Expression system for standard: NS0; Immunogen sequence: H16-F497
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse Angiopoietin-1/ANGPT1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1296)
背景资料
Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1296-CAP | 96-well plate precoated with anti- mouse Angiopoietin-1 antibody | 1 |
EK1296-ST | Lyophilized recombinant mouse Angiopoietin-1 standard | 10ng/tube×2 |
EK1296-DA | Biotinylated anti- mouse Angiopoietin-1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 351 | 1479 | 5183 | 351 | 1592 | 4701 |
Standard deviation | 27.02 | 97.61 | 207.32 | 30.53 | 117.8 | 230.34 |
CV(%) | 7.7% | 6.6% | 4% | 8.7% | 7.4% | 4.9% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.073 | 0.107 | 0.161 | 0.226 | 0.424 | 0.779 | 1.405 | 2.238 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)