Mouse Angiopoietin-1/ANGPT1 ELISA Kit

96T/2480

AGP1; AGPT; ANG 1; ANG1; angiopoietin 1; Angiopoietin-1; ANGPT1; KIAA0003

Expression system for standard: NS0; Immunogen sequence: H16-F497

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

156pg/ml-10000pg/ml

ANGPT1

Angiopoietin-1

O08538

Mouse Angiopoietin-1/ANGPT1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1296)

Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.

Catalog number	Description	Quantity
EK1296-CAP	96-well plate precoated with anti- mouse Angiopoietin-1 antibody	1
EK1296-ST	Lyophilized recombinant mouse Angiopoietin-1 standard	10ng/tube×2
EK1296-DA	Biotinylated anti- mouse Angiopoietin-1 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	351	1479	5183	351	1592	4701
Standard deviation	27.02	97.61	207.32	30.53	117.8	230.34
CV(%)	7.7%	6.6%	4%	8.7%	7.4%	4.9%

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.073	0.107	0.161	0.226	0.424	0.779	1.405	2.238

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h