产品名称
Human CD32b/c ELISA Kit
指标别名
CD32; CD32B; CDw32; Fc gamma RII b; Fc gamma RIIb; FCG2; FCGR2; FCGR2B; FcRII b; IGFR2; IgG Fc receptor II b; IgG Fc receptor II-c; CDw32; Fc-gamma RII-c; Fc-gamma-RIIc; FcRII-c; FCGR2C; IGFR2
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
46.9 pg/ml - 3000 pg/ml
基因名全称
Fc fragment of IgG, low affinity IIb, receptor (CD32)
文献引用格式
Human CD32b/c ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1312)
背景资料
CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1312-CAP | 96-well plate precoated with anti- human CD32 antibody | 1 |
EK1312-ST | Lyophilized recombinant human CD32 standard | 10ng/tube×2 |
EK1312-DA | Biotinylated anti- human CD32 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 122 | 404 | 1372 | 133 | 365 | 1478 |
Standard deviation | 8.05 | 19.79 | 97.41 | 9.7 | 22.26 | 130.06 |
CV(%) | 6.6 | 4.9 | 7.1 | 7.3 | 6.1 | 8.8 |
典型数据示例
Concentration(pg/ml) | 0 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 |
O.D. | 0.007 | 0.124 | 0.199 | 0.255 | 0.541 | 0.813 | 1.408 | 2.266 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)