产品名称
Human ADAMTS1 ELISA Kit
指标别名
ADAM TS 1; ADAM TS1; ADAMTS 1; ADAMTS1; C3 C5; KIAA1346; METH 1; METH1
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
93.8 pg/ml - 6000 pg/ml
基因名全称
ADAM metallopeptidase with thrombospondin type 1 motif, 1
文献引用格式
Human ADAMTS1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1340)
背景资料
ADAMTS1 (A Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif, 1), also known as METH1, is an enzyme that in humans is encoded by the ADAMTS1 gene. ADAMTS is a family of proteins believed to be anchored to the extracellular matrix (ECM) through interactions with aggregan or other matrix components by one or more thrombospondin type 1 motifs. ADAMTS1 gene is mapped to 21q21.2 based on sequence similarity between the ADAMTS1 sequence and a chromosome 21q21.2 clone. It has been found that ADAMTS1 can disrupt angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin. What’s more, ADAMTS1 disrupts angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1340-CAP | 96-well plate precoated with anti- human ADAMTS1 antibody | 1 |
EK1340-ST | Lyophilized recombinant human ADAMTS1 standard | 10ng/tube×2 |
EK1340-DA | Biotinylated anti- human ADAMTS1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 145 | 681 | 2886 | 132 | 732 | 2843 |
Standard deviation | 8.84 | 37.45 | 207.79 | 10.56 | 51.97 | 213.22 |
CV(%) | 6.1 | 5.5 | 7.2 | 8 | 7.1 | 7.5 |
典型数据示例
Concentration(pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
O.D. | 0.026 | 0.096 | 0.160 | 0.278 | 0.513 | 0.920 | 1.599 | 2.476 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin)