产品名称
Human Livin/BIRC7 ELISA Kit
指标别名
BIRC7; KIAP; LIVIN; ML IAP; MLIAP; RING finger protein 50; RNF50
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
baculoviral IAP repeat containing 7
文献引用格式
Human Livin/BIRC7 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1348)
背景资料
BIRC7, also called KIAP or Livin, is a protein that in humans is encoded by the BIRC7 gene. This gene is mapped to 20q13.33. It encodes a member of the inhibitor of apoptosis protein (IAP) family, and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Elevated levels of the encoded protein may be associated with cancer progression and play a role in chemotherapy sensitivity. BIRC7 blocked apoptosis induced by BAX induction of cytochrome C release from mitochondria, and it also can block apoptosis induced by chemotherapeutic agents.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1348-CAP | 96-well plate precoated with anti- human Livin antibody | 1 |
EK1348-ST | Lyophilized recombinant human Livin standard | 100ng/tube×2 |
EK1348-DA | Biotinylated anti- human Livin antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1179 | 7213 | 23834 | 1141 | 6671 | 22267 |
Standard deviation | 75.45 | 310.15 | 1430.04 | 76.44 | 353.56 | 1625.49 |
CV(%) | 6.4 | 4.3 | 6 | 6.7 | 5.3 | 7.3 |
典型数据示例
Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
O.D. | 0.179 | 0.230 | 0.259 | 0.323 | 0.474 | 0.817 | 1.264 | 1.825 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Cell Lysates