产品名称
Human ADAMTS4 ELISA Kit
指标别名
ADAM TS 4; ADAM TS4; ADAMTS 2; ADAMTS 4; ADAMTS4; ADMP 1; Aggrecanase 1; KIAA0688
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
625 pg/ml - 40000 pg/ml
基因名全称
ADAM metallopeptidase with thrombospondin type 1 motif, 4
文献引用格式
Human ADAMTS4 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1372)
背景资料
ADAMTS4, A disintegrin and metalloproteinase with thrombospondin motifs 4 is an enzyme that in humans is encoded by the ADAMTS4 gene. ADAMTS4 is a member of the large ADAMTS family of zinc-dependent proteases. The human ADAMTS4 gene is mapped to chromosome 1 by somatic cell hybrid analysis. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1372-CAP | 96-well plate precoated with anti- human ADAMTS4 antibody | 1 |
EK1372-ST | Lyophilized recombinant human ADAMTS4 standard | 40ng/tube×2 |
EK1372-DA | Biotinylated anti- human ADAMTS4 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1127 | 4290 | 29063 | 1210 | 4672 | 29313 |
Standard deviation | 46.2 | 184.47 | 1627.52 | 71.39 | 200.89 | 2081.22 |
CV(%) | 4.1 | 4.3 | 5.6 | 5.9 | 4.3 | 7.1 |
典型数据示例
Concentration(pg/ml) | 0 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 | 40000 |
O.D. | 0.025 | 0.106 | 0.182 | 0.301 | 0.535 | 1.005 | 1.655 | 2.265 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)