Human ANGPTL3 ELISA Kit

96T/2480

ANG 5; Angiopoietin 5; angiopoietin like 3; Angiopoietin like protein 3; Angiopoietin related protein 3; ANGPT5; ANGPTL3

sf21, S17-E460

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

312 pg/ml - 20000 pg/ml

ANGPTL3

angiopoietin-like 3

Q9Y5C1

Human ANGPTL3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1374)

ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN)-like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.

15-20分钟

Catalog number	Description	Quantity
EK1374-CAP	96-well plate precoated with anti- human ANGPTL3 antibody	1
EK1374-ST	Lyophilized recombinant human ANGPTL3 standard	20ng/tube×2
EK1374-DA	Biotinylated anti- human ANGPTL3 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	704	1869	9766	766	1705	9664
Standard deviation	32.38	110.27	556.66	46.72	121.05	570.17
CV(%)	4.6	5.9	5.7	6.1	7.1	5.9

Concentration(pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.096	0.220	0.286	0.505	0.814	1.279	1.970	2.589

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h