| Catalog number | Description | Quantity |
|---|---|---|
| EK1472-CAP | 96-well plate precoated with anti- human SFRP5 antibody | 1 |
| EK1472-ST | Lyophilized recombinant human SFRP5 standard | 10ng/tube×2 |
| EK1472-DA | Biotinylated anti- human SFRP5 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 329 | 1068 | 4969 | 309 | 1159 | 5218 |
| Standard deviation | 20.39 | 69.42 | 203.72 | 21.93 | 96.19 | 250.46 |
| CV(%) | 6.2 | 6.5 | 4.1 | 7.1 | 8.3 | 4.8 |
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.056 | 0.168 | 0.294 | 0.516 | 0.953 | 1.487 | 1.912 | 2.189 |

Human SFRP5 ELISA Kit standard curve

Human SFRP5 ELISA Kit standard curve


