产品名称
Human ADAM15 ELISA Kit
指标别名
ADAM 15; ADAM15; MDC 15; MDC15; Metargidin
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4000 pg/ml
基因名全称
ADAM metallopeptidase domain 15
文献引用格式
Human ADAM15 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1484)
背景资料
Disintegrin and metalloproteinase domain-containing protein 15 is an enzyme that in humans is encoded by the ADAM15 gene. The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as an EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1484-CAP | 96-well plate precoated with anti- human ADAM15 antibody | 1 |
EK1484-ST | Lyophilized recombinant human ADAM15 standard | 10ng/tube×2 |
EK1484-DA | Biotinylated anti- human ADAM15 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 112 | 674 | 1454 | 110 | 704 | 1511 |
Standard deviation | 5.04 | 53.24 | 59.61 | 7.04 | 64.06 | 64.97 |
CV(%) | 4.5 | 7.9 | 4.1 | 6.4 | 9.1 | 4.3 |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.061 | 0.083 | 0.109 | 0.185 | 0.404 | 0.664 | 1.373 | 2.079 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)