Catalog number | Description | Quantity |
---|---|---|
EK1487-CAP | 96-well plate precoated with anti- human Afamin antibody | 1 |
EK1487-ST | Lyophilized recombinant human Afamin standard | 2ng/tube×2 |
EK1487-DA | Biotinylated anti- human Afamin antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 54 | 287 | 838 | 49 | 308 | 783 |
Standard deviation | 2.75 | 18.94 | 61.17 | 2.84 | 25.87 | 60.29 |
CV(%) | 5.1 | 6.6 | 7.3 | 5.8 | 8.4 | 7.7 |
Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.030 | 0.149 | 0.251 | 0.480 | 0.751 | 1.261 | 1.806 | 2.089 |
Human Afamin ELISA Kit standard curve
Human Afamin ELISA Kit standard curve