产品名称
Mouse ALK-1/ACVRL1 ELISA Kit
指标别名
Activin receptor like kinase 1; ACVRL1; ACVRLK1; ALK 1; ALK1; HHT; HHT2; ORW2; SKR3; TSR I
免疫原
Expression system for standard: NS0; Immunogen sequence: D23-P119
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Serine/threonine-protein kinase receptor R3
文献引用格式
Mouse ALK-1/ACVRL1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1519)
背景资料
Serine/threonine-protein kinase receptor R3 is an enzyme that in humans is encoded by the ACVRL1 gene. It is also known as activin receptor-like kinase 1, or ALK1. This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1519-CAP | 96-well plate precoated with anti- mouse ALK-1 antibody | 1 |
EK1519-ST | Lyophilized recombinant mouse ALK-1 standard | 10ng/tube×2 |
EK1519-DA | Biotinylated anti- mouse ALK-1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 92 | 338 | 1289 | 84 | 319 | 1165 |
Standard deviation | 6.8 | 23.66 | 101.83 | 6.63 | 27.43 | 93.2 |
CV(%) | 7.4% | 7% | 7.9% | 7.9% | 8.6% | 8% |
典型数据示例
Concentration(pg/ml) | 0 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 |
O.D. | 0.032 | 0.060 | 0.138 | 0.239 | 0.538 | 1.045 | 1.895 | 2.280 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)