Human ARMETL1/CDNF ELISA Kit

96T/2800

ARMET like protein 1; ARMETL1; cdnf

E.coli,Q25-L187

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

15.6 pg/ml - 1000 pg/ml

CDNF

cerebral dopamine neurotrophic factor

Q49AH0  

Human ARMETL1/CDNF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1656)

Cerebral dopamine neurotrophic factor also known as ARMET-like protein 1 or is a protein that in humans that is encoded by the CDNF gene. CDNF and MANF are secreted proteins with 8 conserved cysteine residues, which predict a unique protein fold and define an evolutionarily conserved protein family. The human CDNF mRNA encodes a 187-amino acid protein with a predicted signal peptide and apparently no prosequence. The predicted secondary structure, like that of MANF, is dominated by alpha-helices. Human CDNF shares 59% amino acid identity with human MANF, and 49% and 46% identity with D. melanogaster and C. elegans Manf proteins, respectively. RT-PCR of mouse brain detected Cdnf transcripts in embryonic and postnatal stages, and in adult brain regions, including the striatum. In the midbrain, Cdnf mRNA was expressed from embryonic stages to the adult. High levels of Cdnf mRNA were observed in adult mouse heart, skeletal muscle, and testis. In agreement with the results from mouse, wide expression of CDNF transcripts was detected in human brain and nonneuronal tissues. Interestingly, levels of CDNF transcripts were relatively high in the corpus callosum and optic nerve, which are devoid of neuronal cell bodies and contain mostly axonal projections and oligodendrocytes. At postnatal stage P10 of the mouse brain, Cdnf staining was most intense in the hippocampus and thalamus. Cdnf signal was also detected in striatum and substantia nigra.

25-30分钟

Catalog number	Description	Quantity
EK1656-CAP	96-well plate precoated with anti- human CDNF antibody	1
EK1656-ST	Lyophilized recombinant human CDNF standard	10ng/tube×2
EK1656-DA	Biotinylated anti- human CDNF antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	30	220	491	27	208	458
Standard deviation	1.44	10.56	33.38	1.37	10.6	33.43
CV(%)	4.8	4.8	6.8	5.1	5.1	7.3

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.008	0.050	0.101	0.167	0.363	0.602	1.296	2.357

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)

3.5h