Mouse Apolipoprotein H/APOH ELISA Kit

96T/2800

Anticardiolipin cofactor; APC inhibitor; Apo H; APOH; Apolipoprotein H; B2G1; B2GPI; Beta 2 glycoprotein 1; Beta 2 glycoprotein I; BG

Expression system for standard: NS0; Immunogen sequence: G20-C345

There is no detectable cross-reactivity with other relevant proteins.

<50pg/ml

312pg/ml-20000pg/ml

Apoh

apolipoprotein H

Q01339

Mouse Apolipoprotein H/APOH ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK2027)

Apolipoprotein H (Apo-H), previously known as β2-glycoprotein I and beta-2 glycoprotein I, is a 38 kDa multifunctional apolipoprotein that in humans is encoded by the APOH gene. This gene is mapped to 11 E1; 11 71.8 Cm. Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections.

Catalog number	Description	Quantity
EK2027-CAP	96-well plate precoated with anti- mouse APOH antibody	1
EK2027-ST	Lyophilized recombinant mouse APOH standard	20ng/tube×2
EK2027-DA	Biotinylated anti- mouse APOH antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	376	2458	8756	384	2487	8819
Standard deviation	15.04	140.11	402.78	19.58	171.60	617.33
CV(%)	4.0%	5.7%	4.6%	5.1%	6.9%	7.0%

Concentration(pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.034	0.098	0.150	0.303	0.486	0.951	1.628	2.289

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)

3.5h