产品名称
Mouse Apolipoprotein H/APOH ELISA Kit
指标别名
Anticardiolipin cofactor; APC inhibitor; Apo H; APOH; Apolipoprotein H; B2G1; B2GPI; Beta 2 glycoprotein 1; Beta 2 glycoprotein I; BG
免疫原
Expression system for standard: NS0; Immunogen sequence: G20-C345
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse Apolipoprotein H/APOH ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK2027)
背景资料
Apolipoprotein H (Apo-H), previously known as β2-glycoprotein I and beta-2 glycoprotein I, is a 38 kDa multifunctional apolipoprotein that in humans is encoded by the APOH gene. This gene is mapped to 11 E1; 11 71.8 Cm. Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK2027-CAP | 96-well plate precoated with anti- mouse APOH antibody | 1 |
EK2027-ST | Lyophilized recombinant mouse APOH standard | 20ng/tube×2 |
EK2027-DA | Biotinylated anti- mouse APOH antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 376 | 2458 | 8756 | 384 | 2487 | 8819 |
Standard deviation | 15.04 | 140.11 | 402.78 | 19.58 | 171.60 | 617.33 |
CV(%) | 4.0% | 5.7% | 4.6% | 5.1% | 6.9% | 7.0% |
典型数据示例
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.034 | 0.098 | 0.150 | 0.303 | 0.486 | 0.951 | 1.628 | 2.289 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)