产品名称
Mouse Cadherin-4/CDH4 ELISA Kit
指标别名
CAD4; Cadherin 4; CDH4; R CAD; RCAD; R-cadherin; Retinal cadherin
免疫原
Expression system for standard: NS0; Immunogen sequence: D167-A731
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse Cadherin-4/CDH4 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK2094)
背景资料
Cadherin-4 is a protein that in humans is encoded by the CDH4 gene. It is mapped to 2 H4; 2 101.86 cM. This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion and regulate many morphogenetic events during development. The encoded preproprotein is further processed to generate a mature protein. The encoded protein is involved in retinal angiogenesis during development where it plays a crucial role in the endothelial-astrocyte interactions. Alternative splicing results in multiple transcript variants encoding different isoforms.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK2094-CAP | 96-well plate precoated with anti- mouse Cadherin-4/R-Cadherin antibody | 1 |
EK2094-ST | Lyophilized recombinant mouse Cadherin-4/R-Cadherin standard | 10ng/tube×2 |
EK2094-DA | Biotinylated anti- mouse Cadherin-4/R-Cadherin antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 193 | 781 | 4510 | 202 | 871 | 4643 |
Standard deviation | 9.26 | 40.61 | 243.54 | 10.30 | 47.91 | 292.51 |
CV(%) | 4.8% | 5.2% | 5.4% | 5.1% | 5.5% | 6.3% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.058 | 0.156 | 0.275 | 0.464 | 0.756 | 0.981 | 1.430 | 1.754 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)