产品名称
Mouse IFN Beta/IFNB1 ELISA Kit
指标别名
Fibroblast IFN; IFB; IFF; IFN beta; IFNB; IFNB1; IFN-beta
免疫原
Expression system for standard: HEK293; Immunogen sequence: I22-N182
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4,000 pg/ml
基因名全称
interferon beta 1, fibroblast
背景资料
Interferon beta is a protein that in humans is encoded by the IFNB1 gene. This gene encodes a cytokine that belongs to the interferon family of signaling proteins, which are released as part of the innate immune response to pathogens. The protein encoded by this gene belongs to the type I class of interferons, which are important for defense against viral infections. In addition, type I interferons are involved in cell differentiation and anti-tumor defenses. Following secretion in response to a pathogen, type I interferons bind a homologous receptor complex and induce transcription of genes such as those encoding inflammatory cytokines and chemokines. Overactivation of type I interferon secretion is linked to autoimmune diseases. Mice deficient for this gene display several phenotypes including defects in B cell maturation and increased susceptibility to viral infection.
试剂盒内容
Catalog number | Description | Quantity |
EK2285-CAP | 96-well plate precoated with anti- Mouse IFN Beta/IFNB1 ELISA Kit A antibody | 1 |
EK2285-ST | Lyophilized recombinant Mouse IFN Beta/IFNB1 ELISA Kit A standard | 4ng/tube×2 |
EK2285 -DA | Biotinylated anti- Mouse IFN Beta/IFNB1 ELISA Kit A antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 72 | 354 | 1168 | 78 | 459 | 1393 |
Standard deviation | 5.66 | 22.62 | 67.98 | 3.08 | 36.72 | 91.8 |
CV(%) | 7.9% | 6.4% | 5.8% | 4% | 8% | 6.6% |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 | 4,000 |
O.D. | 0.033 | 0.078 | 0.149 | 0.276 | 0.441 | 0.784 | 1.442 | 1.941 |
保存条件与有效期
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum.