Human Laminin EZ-Set™ ELISA Kit (DIY Antibody Pairs)

5 plates(kit)/3980

Laminin subunit alpha-1, Laminin A chain, Laminin-1 subunit alpha, Laminin-3 subunit alpha, S-laminin subunit alpha (S-LAM alpha), LAMA1, LAMA, Laminin subunit alpha-2, Laminin M chain, Laminin-12 subunit alpha, Laminin-2 subunit alpha, Laminin-4 subunit alpha, Merosin heavy chain, LAMA2, LAMM, Laminin subunit alpha-3, Epiligrin 170 kDa subunit (E170), Epiligrin subunit alpha, Kalinin subunit alpha, Laminin-5 subunit alpha, Laminin-6 subunit alpha, Laminin-7 subunit alpha, Nicein subunit alpha, LAMA3, LAMNA, Laminin subunit alpha-4, Laminin-14 subunit alpha, Laminin-8 subunit alpha, Laminin-9 subunit alpha, LAMA4, Laminin subunit alpha-5, Laminin-10 subunit alpha, Laminin-11 subunit alpha, Laminin-15 subunit alpha, LAMA5, KIAA0533, KIAA1907, Laminin subunit beta-1, Laminin B1 chain, Laminin-1 subunit beta, Laminin-10 subunit beta, Laminin-12 subunit beta, Laminin-2 subunit beta, Laminin-6 subunit beta, Laminin-8 subunit beta, LAMB1, Laminin subunit beta-2, Laminin B1s chain, Laminin-11 subunit beta, Laminin-14 subunit beta, Laminin-15 subunit beta, Laminin-3 subunit beta, Laminin-4 subunit beta, Laminin-7 subunit beta, Laminin-9 subunit beta, S-laminin subunit beta (S-LAM beta), Laminin subunit beta-3, Epiligrin subunit bata, Kalinin B1 chain, Kalinin subunit beta, Laminin B1k chain, Laminin-5 subunit beta, Nicein subunit beta, LAMB3, LAMNB1, Laminin subunit beta-4, Laminin beta-1-related protein, LAMB4, Laminin subunit gamma-1,Laminin B2 chain, Laminin-1 subunit gamma, Laminin-10 subunit gamma, Laminin-11 subunit gamma, Laminin-2 subunit gamma, Laminin-3 subunit gamma, Laminin-4 subunit gamma, Laminin-6 subunit gamma, Laminin-7 subunit gamma, Laminin-8 subunit gamma, Laminin-9 subunit gamma, S-laminin subunit gamma (S-LAM gamma), LAMC1, LAMB2, Laminin subunit gamma-2, Cell-scattering factor 140 kDa subunit (CSF 140 kDa subunit), Epiligrin subunit gamma, Kalinin subunit gamma, Kalinin/nicein/epiligrin 100 kDa subunit, Ladsin 140 kDa subunit, Laminin B2t chain, Laminin-5 subunit gamma, Large adhesive scatter factor 140 kDa subunit, Nicein subunit gamma, LAMC2, LAMB2T, LAMNB2, Laminin subunit gamma-3, Laminin-12 subunit gamma, Laminin-14 subunit gamma, Laminin-15 subunit gamma, LAMC3

Expression system for standard: from human fibroblasts

There is no detectable cross-reactivity with other relevant proteins.

156 pg/ml - 10,000 pg/ml

LAMA1/A2/A3/A4/A5/B1/B2/B3/B4/C1/C2/C3

laminin subunit alpha 1, laminin subunit alpha 2, laminin subunit alpha 3, laminin subunit alpha 4, laminin subunit alpha 5, laminin subunit beta 1, laminin subunit beta 2, laminin subunit beta 3, laminin subunit beta 4, laminin subunit gamma 1, laminin subunit gamma 2, laminin subunit gamma 3

P25391/P24043/Q16787/Q16363/O15230/P07942/P55268/Q13751/A4D0S4/P11047/Q13753/Q9Y6N6  

Human Laminin EZ-Set™ ELISA Kit (DIY Antibody Pairs) (Boster Biological Technology, Wuhan, China. Catalog#EZ0434)

Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2.1 Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3. The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide.2 The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology. The human laminin B2 chain gene maps to the long arm of chromosome 1 in the band q31.

Catalog number	Description	Quantity	Storage of opened/reconstituted material
EZ0434-CA	Rabbit anti- human Laminin polyclonal antibody (Capture Antibody)	500 µl, 0.4 mg/mL (recommended dilution 1:100)	Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ0434-DA	Biotinylated goat anti- human Laminin polyclonal antibody (Detection Antibody)	500 µl, 30 µg/mL (recommended dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	500 µl (recommended dilution 1:100)
EZ0434-ST	Lyophilized recombinant human Laminin standard	10 ng/tube×3	Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.095	0.137	0.168	0.247	0.425	0.624	1.284	2.010

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution:
Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12.Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
*样品准备的相关试剂也不含在试剂盒中。

human

Laminin subunit alpha-1, Laminin subunit alpha-2, Laminin subunit alpha-3, Laminin subunit alpha-4, Laminin subunit alpha-5, Laminin subunit beta-1, Laminin subunit beta-2, Laminin subunit beta-3, Laminin subunit beta-4, Laminin subunit gamma-1, Laminin subunit gamma-2, Laminin subunit gamma-3

Cell Culture Supernatants, Cell Lysates and Serum