产品名称
Mouse IL17F EZ-Set™ ELISA Kit (DIY Antibody Pairs)
指标别名
Cytokine ML 1; IL 17F; IL17F; IL-17F; interleukin 17F; ML 1; ML1
免疫原
Expression system for standard: E.coli; Immunogen sequence: R21-A153
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2,000 pg/ml
文献引用格式
Mouse IL17F EZ-Set™ ELISA Kit (DIY Antibody Pairs) (Boster Biological Technology, Wuhan, China. Catalog#EZ0796)
背景资料
Interleukin 17F, also called IL17F is involved in the regulation of normal versus aberrant T-cell responses. This gene is mapped to 6p12.2. The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. It is suggested that targeting IL17 and IL17F or antagonizing IL17R might mitigate neutrophil-mediated inflammation in CF.
试剂盒内容
| Catalog number |
Description |
Quantity |
Storage of opened/reconstituted material |
| EZ0796-CA |
Mouse anti- mouse Il17f monoclonal antibody (Capture Antibody) |
500 µl, 4 mg/mL
(recommended dilution 1:100) |
Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
|
| EZ0796-DA |
Biotinylated goat anti- mouse Il17f polyclonal antibody (Detection Antibody) |
500 µl, 0.15 µg/mL
(recommended dilution 1:100) |
| AR1103 |
Avidin-Biotin-Peroxidase Complex (ABC) |
500 µl (recommended dilution 1:100) |
| EZ0796-ST |
Lyophilized recombinant mouse Il17f standard |
10 ng/tube×3
|
Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit. |
典型数据示例
| Concentration (pg/ml) | 0 | 31.25 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.003 | 0.067 | 0.14 | 0.282 | 0.585 | 1.12 | 1.76 | 2.209 |
保存条件与有效期
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
需要自备的试剂
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution:
Tetramethylbenzidine (Cat# AR1104)
11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12.Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
*样品准备的相关试剂也不含在试剂盒中。
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)
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