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Human CRP EZ-Set™ ELISA Kit (DIY Antibody Pairs)

说明书

EZ1316

  • 5 plates(kit) ¥3980
  • 货期: 3-5天
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产品名称
Human CRP EZ-Set™ ELISA Kit (DIY Antibody Pairs)
规格/价格
5 plates(kit)/3980
指标别名
C reactive protein; CRP; PTX1
免疫原
Expression system for standard: NS0; Immunogen sequence: F17-P224
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
312 pg/ml - 20,000 pg/ml
基因名称
CRP
基因名全称
C-reactive protein, pentraxin-related
Uniprot ID
P02741  
文献引用格式
Human CRP EZ-Set™ ELISA Kit (DIY Antibody Pairs) (Boster Biological Technology, Wuhan, China. Catalog#EZ1316)
背景资料
C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.
试剂盒内容
Catalog number Description Quantity Storage of opened/reconstituted material
EZ1316-CA Rabbit anti- human CRP polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ1316-DA Biotinylated goat anti- human CRP polyclonal antibody (Detection Antibody) 500 µl, 3.2 µg/mL (recommended dilution 1:100)
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)
EZ1316-ST Lyophilized recombinant human CRP standard 20 ng/tube×3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.
典型数据示例
Concentration (pg/ml)03126251250250050001000020000
O.D.0.0050.0460.10.1740.4480.831.3741.959
保存条件与有效期
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
需要自备的试剂
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution:
Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12.Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
*样品准备的相关试剂也不含在试剂盒中。
检验物种
human
蛋白名全称
C-reactive protein
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

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