产品名称
Mouse EGF Quick ELISA Kit
指标别名
EGF; HOMG4; Pro epidermal growth factor; URG; Urogastrone
免疫原
Expression system for standard: E.coli; Immunogen sequence: N977-R1029
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
epidermal growth factor
文献引用格式
Mouse EGF Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0326)
背景资料
Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. EGF acts by binding with high affinity to epidermal growth factor receptor(EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. EGF results in cellular proliferation, differentiation, and survival. It also has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. EGF has strong expression in kidney, salivary gland, cerebrum, and prostate, moderate expression in trachea and thyroid, and low expression in bone marrow, heart, spleen, thymus, uterus, and colon. No expression was detected in adrenal gland, liver, lung, cerebellum, placenta, and small intestine.
试剂盒内容
| Catalog number | Description | Quantity |
| FEK0326-CAP | Anti-Pre-coated 96-well Strip Microplate | 1 |
| FEK0326-ST | Mouse EGF standard | 10ng/tube×2 |
| FEK0326-A | Mouse EGF Antibody A | 3ml |
| FEK0326-B | Mouse EGF Antibody B | 3ml |
| AR1106-1 | Sample diluent buffer | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | Stop solution | 10ml |
| PLA-SEA | Adhesive cover | 2 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 18 | 52 | 277 | 19 | 63 | 274 |
| Standard deviation | 1.35 | 3.43 | 15.24 | 0.97 | 5.1 | 18.08 |
| CV(%) | 7.5% | 6.6% | 5.5% | 5.1% | 8.1% | 6.6% |
典型数据示例
| Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.045 | 0.190 | 0.322 | 0.554 | 0.989 | 1.746 | 2.328 | 2.403 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Urine
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