产品名称
Human Adiponectin/ADIPOQ Quick ELISA Kit
指标别名
ACDC; ACRP30; adiponectin; ADIPOQ; ADIPQTL1; ADPN; APM 1; APM1; GBP28; Gelatin binding protein
免疫原
Expression system for standard: NS0; Immunogen sequence: E19-N244
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
adiponectin, C1Q and collagen domain containing
文献引用格式
Human Adiponectin/ADIPOQ Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0595)
背景资料
Adiponectin(ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin. The standard product used in this kit is the product of gene recombination, consisting of 226(19-244) amino acids with the molecular mass of 36KDa after glycosylation.
试剂盒内容
| Catalog number | Description | Quantity |
| FEK0595-CAP | Anti-Pre-coated 96-well Strip Microplate | 1 |
| FEK0595-ST | Human Adiponectin standard | 10ng/tube×2 |
| FEK0595-A | Human Adiponectin Antibody A | 3ml |
| FEK0595-B | Human Adiponectin Antibody B | 3ml |
| AR1106-1 | Sample diluent buffer | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | Stop solution | 10ml |
| PLA-SEA | Adhesive cover | 2 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 3726 | 12058 | 49927 | 3646 | 11478 | 49590 |
| Standard deviation | 182.57 | 940.52 | 2346.56 | 236.99 | 1033.02 | 2578.68 |
| CV(%) | 4.9% | 7.8% | 4.7% | 6.5% | 9% | 5.2% |
典型数据示例
| Concentration(ng/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D. | 0.038 | 0.081 | 0.169 | 0.341 | 0.633 | 1.020 | 1.845 | 2.537 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Urine
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