Anti-BRAF Antibody (Clone#OTI5A9)

Mouse monoclonal antibody

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筛选器： All WB IHC ICC/IF FCM

M00075-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of extracts (35ug) from 4 tissue lysates by using anti-BRAF monoclonal antibody (1:500).
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and BRAF-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-BRAF monoclonal antibody M00075-3 (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BRAF monoclonal antibody.
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF(Cat# M00075-3).
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BRAF mouse monoclonal antibody. (M00075-3)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
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Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-BRAF mouse monoclonal antibody. (M00075-3)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
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Anti-BRAF mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BRAF .
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HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BRAF antibody, and then analyzed by flow cytometry.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-BRAF Antibody (Clone#OTI5A9)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

B RAF1; BRAF; BRAF1; p94; Proto oncogene B Raf; RAFB1

产品类型

Monoclonal

检验物种

human, mouse, rat, monkey

应用范围

WB, IHC, ICC/IF, FCM

基因名称

BRAF

克隆号

OTI5A9

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human BRAF (NP_004324) produced in HEK293T cell.

计算分子量

84 kDa

实际分子量

85 kDa

成分

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a protein called B-Raf. It is a member of the Raf kinase family of growth signal transduction protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. It is mapped to 7q34. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. The BRAF protein is also involved in sending signals inside cells, which are involved in directing cell growth.

Uniprot ID

P15056

文献引用格式

BRAF Antibody (Clone#OTI5A9) (Boster Biological Technology, Wuhan, China. Catalog#M00075-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

v-raf murine sarcoma viral oncogene homolog B1

蛋白名全称

Serine/threonine-protein kinase B-raf

实验方案>

实验步骤

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