Anti-B7-1/CD80 Antibody (Clone#OTI6H7)

Mouse monoclonal antibody

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筛选器： All WB ICC/IF FCM

M00196-4

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-B7-1/CD80 antibody (M00196-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-B7-1/CD80 antigen affinity purified monoclonal antibody (M00196-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for B7-1/CD80 at approximately 30 kDa. The expected band size for B7-1/CD80 is at 35 kDa.
all(3)
Anti-CD80 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD80 .
all(3)
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CD80 antibody, and then analyzed by flow cytometry.
all(3)

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产品名称

Anti-B7-1/CD80 Antibody (Clone#OTI6H7)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Activation B7 1 antigen; B7; B7 1; B7-1; BB1; CD28LG; CD28LG1; CD80; CD80 molecule; CTLA 4 counter receptor B7.1; LAB7

产品类型

Monoclonal

检验物种

human

应用范围

WB, ICC/IF, FCM

基因名称

CD80

克隆号

OTI6H7

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human CD80 (NP_005182) produced in HEK293T cell.

计算分子量

35 kDa

实际分子量

29.3 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

Cluster of Differentiation 80 (also CD80 and B7-1) is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. The CD80 genes encode B7-1 which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types.

Uniprot ID

Q00609

文献引用格式

B7-1/CD80 Antibody (Clone#OTI6H7) (Boster Biological Technology, Wuhan, China. Catalog#M00196-4)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

CD80 antigen

蛋白名全称

T-lymphocyte activation antigen CD80

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实验步骤

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