Figure 1. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
all(7)
Figure 2. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SQSTM1 antibody, and then analyzed by flow cytometry.
all(7) 
Figure 3. Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00300-4)
all(7) 
Figure 4. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00300-4)
all(7) 
Figure 5. Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00300-4)
all(7) 
Figure 6. Anti-SQSTM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1 .
all(7) 
Figure 7. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1(Cat# M00300-4).
all(7) | Western blot (WB): | 1:500~2000 |
| Immunohistochemistry (IHC): | 1:150 |
| Immunofluorescence (IF): | 1:100 |
| Flow cytometry (FCM): | 1:100 |
Figure 1. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Figure 2. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SQSTM1 antibody, and then analyzed by flow cytometry.
Figure 3. Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00300-4)
Figure 4. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00300-4)
Figure 5. Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00300-4)
Figure 6. Anti-SQSTM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1 .
Figure 7. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1(Cat# M00300-4).
Figure 1. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Figure 2. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SQSTM1 antibody, and then analyzed by flow cytometry.
Figure 3. Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00300-4)
Figure 4. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00300-4)
Figure 5. Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00300-4)
Figure 6. Anti-SQSTM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1 .
Figure 7. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1(Cat# M00300-4).
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