| Western blot (WB): | 1:1000 |
| Immunofluorescence (IF): | 1:50~100 |
| Flow cytometry (FCM): | 1:100 |

Figure 1. Immunofluorescent staining of HepG2 cells using anti-PRL mouse monoclonal antibody.

Figure 2. Anti-PRL mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRL .

Figure 3. Flow cytometric Analysis of Jurkat cells, using anti-PRL antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Figure 4. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRL (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRL.

Figure 1. Immunofluorescent staining of HepG2 cells using anti-PRL mouse monoclonal antibody.

Figure 2. Anti-PRL mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRL .

Figure 3. Flow cytometric Analysis of Jurkat cells, using anti-PRL antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Figure 4. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRL (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRL.



