Anti-APEX1 Antibody (Clone#OTI1H9)

筛选器： All WB IHC

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  Figure 1. Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and APEX1-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-APEX1 monoclonal antibody M00627-4, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody ([M01692-2]) as a loading control.

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  Figure 2. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-APEX1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Figure 3. Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00627-4)

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  Figure 4. Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00627-4)

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  Figure 5. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00627-4)

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  Figure 6. Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00627-4)

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  Figure 7. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00627-4)

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  Figure 8. Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00627-4)

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  Figure 9. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APEX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APEX1.

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  Figure 1. Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and APEX1-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-APEX1 monoclonal antibody M00627-4, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody ([M01692-2]) as a loading control.

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  Figure 2. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-APEX1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Figure 3. Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00627-4)

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  Figure 4. Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00627-4)

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  Figure 5. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00627-4)

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  Figure 6. Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00627-4)

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  Figure 7. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00627-4)

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  Figure 8. Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-APEX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00627-4)

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  Figure 9. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APEX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APEX1.