Anti-CYP2E1 Antibody (Clone#2C7G1)

Mouse monoclonal antibody

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筛选器： All WB

M00672-2

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti- CYP2E1 Antibody (M00672-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HCCP tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse liver tissue lysates.
Use mouse anti- CYP2E1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for CYP2E1 at approximately 57KD. The expected band size for CYP2E1 is at 57KD.
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产品名称

Anti-CYP2E1 Antibody (Clone#2C7G1)

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

4 nitrophenol 2 hydroxylase; CPE1; CYP2E; CYP2E1; CYP-2E1; CYP2E1-Specific; CYPIIE1; Cytochrome P450 2E1; Cytochrome P450 J; P450 J; P450C2E

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

基因名称

CYP2E1

克隆号

2C7G1

宿主

Mouse

抗体亚型

IgG2b

免疫原

E.coli-derived human Cytochrome P450 2E1/CYP2E1 recombinant protein (Position: H355-S493).

计算分子量

57 kDa

实际分子量

57 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Cytochrome P450 2E1 (abbreviated CYP2E1), a member of the cytochrome P450 mixed-function oxidase system, is involved in the metabolism of xenobiotics in the body. In humans, the CYP2E1 enzyme is encoded by the CYP2E1 gene. It is mapped to 10q26.3. While it is involved in the oxidative metabolism of a small range of substrates (mostly small polar molecules), there are many important drug interactions mediated by CYP2E1. Most drugs undergo deactivation by CYP2E1, either directly or by facilitated excretion from the body. Also, many substances are bioactivated by CYP2E1 to form their active compounds. In addition, CYP2E1 is an important enzyme for the conversion of ethanol to acetaldehyde and to acetate in humans. In the conversion sequence of acetyl-CoA to glucose, CYP2E1 transforms acetone via acetol into propylene glycol and methylglyoxal, the precursors of pyruvate, acetate and lactate.

Uniprot ID

P05181

文献引用格式

CYP2E1 Antibody (Clone#2C7G1) (Boster Biological Technology, Wuhan, China. Catalog#M00672-2)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

cytochrome P450, family 2, subfamily E, polypeptide 1

蛋白名全称

Cytochrome P450 2E1

实验方案>

实验步骤

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