Anti-PGP9.5/UCHL1 Antibody (Clone#OTI2B5)

Mouse monoclonal antibody

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M01018-7

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-UCHL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01018-7)
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-UCHL1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-UCHL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01018-7)
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HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-UCHL1 antibody, and then analyzed by flow cytometry.
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-UCHL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01018-7)
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UCHL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UCHL1 (Cat# M01018-7).
all(6)

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产品简介>

产品名称

Anti-PGP9.5/UCHL1 Antibody (Clone#OTI2B5)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Neuron cytoplasmic protein 9.5; PARK5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1; Uch L1; UCHL1; UCH-L1; UCHL1/PGP9.5

产品类型

Monoclonal

检验物种

human, mouse, rat, monkey

应用范围

WB, IHC, FCM

基因名称

UCHL1

克隆号

OTI2B5

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human UCHL1(NP_004172) produced in HEK293T cell.

计算分子量

25 kDa

实际分子量

25 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease. [provided by RefSeq, Sep 2009]

Uniprot ID

P09936

文献引用格式

PGP9.5/UCHL1 Antibody (Clone#OTI2B5) (Boster Biological Technology, Wuhan, China. Catalog#M01018-7)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

ubiquitin C-terminal hydrolase L1

蛋白名全称

Ubiquitin carboxyl-terminal hydrolase isozyme L1

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实验步骤

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