Anti-KU70/XRCC6 Antibody (Clone#9B6)

Mouse monoclonal antibody

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筛选器： All WB IHC ICC/IF FCM

M01732-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of KU70/XRCC6 using anti-KU70/XRCC6 antibody (M01732-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-KU70/XRCC6 antigen affinity purified monoclonal antibody (M01732-3) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for KU70/XRCC6 at approximately 70 kDa. The expected band size for KU70/XRCC6 is at 70 kDa.
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IHC analysis of KU70/XRCC6 using anti-KU70/XRCC6 antibody (M01732-3).
KU70/XRCC6 was detected in a paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-KU70/XRCC6 Antibody (M01732-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of KU70/XRCC6 using anti-KU70/XRCC6 antibody (M01732-3).
KU70/XRCC6 was detected in a paraffin-embedded section of human Lymphoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-KU70/XRCC6 Antibody (M01732-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of KU70/XRCC6 using anti-KU70/XRCC6 antibody (M01732-3).
KU70/XRCC6 was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-KU70/XRCC6 Antibody (M01732-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of KU70/XRCC6 using anti-KU70/XRCC6 antibody (M01732-3).
KU70/XRCC6 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-KU70/XRCC6 Antibody (M01732-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IF analysis of KU70/XRCC6 using anti-KU70/XRCC6 antibody (M01732-3).
KU70/XRCC6 was detected in an immunocytochemical section of U2OS cells. The section was incubated with mouse anti-KU70/XRCC6 Antibody (M01732-3) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Flow Cytometry analysis of THP-1 cells using anti-KU70/XRCC6 antibody (M01732-3).
Overlay histogram showing THP-1 cells stained with M01732-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-KU70/XRCC6 Antibody (M01732-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-KU70/XRCC6 Antibody (Clone#9B6)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

5 dRP lyase Ku70; 70 kDa subunit of Ku antigen; CTC75; CTCBF; DNA repair protein XRCC6; G22P1; KU70; ML8; Thyroid lupus autoantigen; TLAA; XRCC6

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, ICC/IF, FCM

基因名称

XRCC6

克隆号

9B6

宿主

Mouse

抗体亚型

IgG2b

免疫原

A synthetic peptide corresponding to a sequence at C-terminus of human Ku70, different from the related mouse sequence by one amino acid.

计算分子量

70 kDa

实际分子量

70 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

XRCC6 (X-Ray Repair, Complementing Defective, In Chinese Hamster, 6), also called Ku70, G22P1 or TLAA, is a protein that in humans, is encoded by the XRCC6 gene. In addition, the XRCC6 gene encodes subunit p70 of the p70/p80 autoantigen which consists of 2 proteins of molecular mass of approximately 70,000 and 80,000 daltons that dimerize to form a 10 S DNA-binding complex. The XRCC6 gene is mapped to 22q13.2. XRCC6 and Mre11 are differentially expressed during meiosis. XRCC6 interacts with Baxa, a mediator of mitochondrial-dependent apoptosis. Disruption of both FANCC and XRCC6 suppressed sensitivity to crosslinking agents, diminished chromosome breaks, and reversed defective homologous recombination. Ku70 binds directly to free DNA ends, committing them to NHEJ repair. In early meiotic prophase, however, when meiotic recombination is most probably initiated, Mre11 was abundant, whereas XRCC6 was not detectable.

Uniprot ID

P12956

文献引用格式

KU70/XRCC6 Antibody (Clone#9B6) (Boster Biological Technology, Wuhan, China. Catalog#M01732-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

X-ray repair complementing defective repair in Chinese hamster cells 6

蛋白名全称

X-ray repair cross-complementing protein 6

实验方案>

实验步骤

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