Figure 1. Western blot analysis of anti- Calpain 1 antibody (M01943-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates,Lane 2: human Hela whole cell lysates,Lane 3: human K562 whole cell lysates,Lane 4: human PC-3 whole cell lysates.Use mouse anti- Calpain 1 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for Calpain 1 at approximately 82KD. The expected band size for Calpain 1 is at 82KD.
all(3)
Figure 2. ICC analysis using anti- Calpain 1 antibody (M01943-3). was detected in immersion fixed A431 cell. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue).
all(3) 
Figure 3. Flow cytometry analysis of A549 cell (1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).
all(3) | Western blot (WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
Figure 1. Western blot analysis of anti- Calpain 1 antibody (M01943-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates,Lane 2: human Hela whole cell lysates,Lane 3: human K562 whole cell lysates,Lane 4: human PC-3 whole cell lysates.Use mouse anti- Calpain 1 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for Calpain 1 at approximately 82KD. The expected band size for Calpain 1 is at 82KD.
Figure 2. ICC analysis using anti- Calpain 1 antibody (M01943-3). was detected in immersion fixed A431 cell. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue).
Figure 3. Flow cytometry analysis of A549 cell (1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).
Figure 1. Western blot analysis of anti- Calpain 1 antibody (M01943-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates,Lane 2: human Hela whole cell lysates,Lane 3: human K562 whole cell lysates,Lane 4: human PC-3 whole cell lysates.Use mouse anti- Calpain 1 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for Calpain 1 at approximately 82KD. The expected band size for Calpain 1 is at 82KD.
Figure 2. ICC analysis using anti- Calpain 1 antibody (M01943-3). was detected in immersion fixed A431 cell. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue).
Figure 3. Flow cytometry analysis of A549 cell (1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).
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