Anti-c-Jun/JUN Antibody (Clone#OTI3G2)

Mouse monoclonal antibody

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筛选器： All WB IHC ICC/IF FCM

M02038-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JUN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JUN.
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IHC analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (M02038-2) .
c-Jun/JUN was detected in a paraffin-embedded section of human cervical cancer tissue. The tissue section was incubated with mouse anti-c-Jun/JUN Antibody (M02038-2) at a dilution of 1:200 and developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (M02038-2) .
c-Jun/JUN was detected in a paraffin-embedded section of human prostate cancer tissue. The tissue section was incubated with mouse anti-c-Jun/JUN Antibody (M02038-2) at a dilution of 1:200 and developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
all(10)
IHC analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (M02038-2) .
c-Jun/JUN was detected in a paraffin-embedded section of human prostate cancer tissue. The tissue section was incubated with mouse anti-c-Jun/JUN Antibody (M02038-2) at a dilution of 1:200 and developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
all(10)
IHC analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (M02038-2) .
c-Jun/JUN was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with mouse anti-c-Jun/JUN Antibody (M02038-2) at a dilution of 1:200 and developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
all(10)
IHC analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (M02038-2) .
c-Jun/JUN was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with mouse anti-c-Jun/JUN Antibody (M02038-2) at a dilution of 1:200 and developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
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Anti-JUN mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY JUN .
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HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-JUN antibody, and then analyzed by flow cytometry.
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Flow cytometric Analysis of Hela cells, using anti-JUN antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-JUN antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-c-Jun/JUN Antibody (Clone#OTI3G2)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Activator protein 1; AP 1; AP1; c Jun; JUN; jun oncogene; P39; Proto oncogene c Jun; Transcription factor AP 1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

JUN

克隆号

OTI3G2

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human JUN (NP_002219) produced in HEK293T cell.

计算分子量

163 kDa

实际分子量

35.5 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq]

Uniprot ID

P05412

文献引用格式

c-Jun/JUN Antibody (Clone#OTI3G2) (Boster Biological Technology, Wuhan, China. Catalog#M02038-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Jun proto-oncogene, AP-1 transcription factor subunit

蛋白名全称

Transcription factor AP-1

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实验步骤

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