Anti-PLA2G2A Antibody (Clone#25P80)

筛选器： All WB

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  Figure 1. Western blot analysis of anti-PLA2G2A antibody (M02259-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human A549 whole cell lysates,
  Lane 2: human HepG2 whole cell lysates,
  Lane 3: human RT4 whole cell lysates,
  Lane 4: human Hacat whole cell lysates,
  Lane 5: rat small intestine tissue lysates,
  Lane 6: rat C6 whole cell lysates,
  Lane 7: mouse small intestine tissue lysates,
  Lane 8: mouse EL-4 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PLA2G2A antigen affinity purified monoclonal antibody (M02259-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PLA2G2A at approximately 14 kDa. The expected band size for PLA2G2A is at 16 kDa.

• 

  Figure 1. Western blot analysis of anti-PLA2G2A antibody (M02259-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human A549 whole cell lysates,
  Lane 2: human HepG2 whole cell lysates,
  Lane 3: human RT4 whole cell lysates,
  Lane 4: human Hacat whole cell lysates,
  Lane 5: rat small intestine tissue lysates,
  Lane 6: rat C6 whole cell lysates,
  Lane 7: mouse small intestine tissue lysates,
  Lane 8: mouse EL-4 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PLA2G2A antigen affinity purified monoclonal antibody (M02259-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PLA2G2A at approximately 14 kDa. The expected band size for PLA2G2A is at 16 kDa.