Anti-PPT1 Antibody (Clone#OTI1A10)

Mouse monoclonal antibody

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筛选器： All WB IHC

M02690-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
all(3)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PPT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02690-1)
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPT1.
all(3)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-PPT1 Antibody (Clone#OTI1A10)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

palmitoyl-protein thioesterase 1; CLN1; INCL; PPT; PPT1; PPT 1

产品类型

Monoclonal

检验物种

human, dog, monkey

应用范围

WB, IHC

基因名称

PPT1

克隆号

OTI1A10

宿主

Mouse

抗体亚型

IgG2b

免疫原

Human recombinant protein fragment corresponding to amino acids 100-306 of human PPT1 (NP_000301) produced in E.coli.

计算分子量

34 kDa

实际分子量

31.2 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

Palmitoyl-protein thioesterase 1 (PPT-1), also known as palmitoyl-protein hydrolase 1, is an enzyme that in humans is encoded by the PPT1 gene. PPT-1 is a member of the palmitoyl protein thioesterase family. The protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene.

Uniprot ID

P50897

文献引用格式

PPT1 Antibody (Clone#OTI1A10) (Boster Biological Technology, Wuhan, China. Catalog#M02690-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

palmitoyl-protein thioesterase 1

蛋白名全称

Palmitoyl-protein thioesterase 1

实验方案>

实验步骤

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