Figure 1. Western blot analysis of anti- MDH2 antibody (M04803-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: U-87MG whole cell lysates,
Lane 3: A431 whole cell lysates.
Use mouse anti- MDH2 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for MDH2 at approximately 36KD. The expected band size for MDH2 is at 36KD.
Figure 2. IHC analysis using anti- MDH2 antibody (M04803-1). detected in paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(5) 
Figure 3. IHC analysis using anti- MDH2 antibody (M04803-1). detected in paraffin-embedded section of human cervical cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(5) 
Figure 4. IHC analysis using anti- MDH2 antibody (M04803-1). detected in paraffin-embedded section of human squamous cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(5) 
Figure 5. ICC analysis using anti- MDH2 antibody (M04803-1). was detected in immersion fixed Hela cell. Cells were stained using the cy3-conjugated Anti- mouse IgG Secondary Antibody (red)(Catalog # BA1031) and counterstained with DAPI (blue)
all(5) | Western blot (WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence(ICC/IF): | 1:50-400 |
| Immunohistochemistry (IHC): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of anti- MDH2 antibody (M04803-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: U-87MG whole cell lysates,
Lane 3: A431 whole cell lysates.
Use mouse anti- MDH2 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for MDH2 at approximately 36KD. The expected band size for MDH2 is at 36KD.
Figure 2. IHC analysis using anti- MDH2 antibody (M04803-1). detected in paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 3. IHC analysis using anti- MDH2 antibody (M04803-1). detected in paraffin-embedded section of human cervical cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 4. IHC analysis using anti- MDH2 antibody (M04803-1). detected in paraffin-embedded section of human squamous cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 5. ICC analysis using anti- MDH2 antibody (M04803-1). was detected in immersion fixed Hela cell. Cells were stained using the cy3-conjugated Anti- mouse IgG Secondary Antibody (red)(Catalog # BA1031) and counterstained with DAPI (blue)
Figure 1. Western blot analysis of anti- MDH2 antibody (M04803-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: U-87MG whole cell lysates,
Lane 3: A431 whole cell lysates.
Use mouse anti- MDH2 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for MDH2 at approximately 36KD. The expected band size for MDH2 is at 36KD.
Figure 2. IHC analysis using anti- MDH2 antibody (M04803-1). detected in paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 3. IHC analysis using anti- MDH2 antibody (M04803-1). detected in paraffin-embedded section of human cervical cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 4. IHC analysis using anti- MDH2 antibody (M04803-1). detected in paraffin-embedded section of human squamous cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Figure 5. ICC analysis using anti- MDH2 antibody (M04803-1). was detected in immersion fixed Hela cell. Cells were stained using the cy3-conjugated Anti- mouse IgG Secondary Antibody (red)(Catalog # BA1031) and counterstained with DAPI (blue)
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