Western blot (WB): | 1:2000 |
Immunohistochemistry (IHC): | 1:2000 |
Figure 1. Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GLS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M05334-1) (1:2000)
Figure 2. Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-GLS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M05334-1) (1:2000)
Figure 3. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLS2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLS2 (1:2000).
Figure 1. Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GLS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M05334-1) (1:2000)
Figure 2. Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-GLS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M05334-1) (1:2000)
Figure 3. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLS2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLS2 (1:2000).