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Anti-GLS2 Antibody (Clone#OTI1C12)

Mouse monoclonal antibody

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筛选器: All IHC WB

M05334-1

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 5-7天
  • Figure 1. Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GLS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M05334-1) (1:2000)

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  • Figure 2. Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-GLS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M05334-1) (1:2000)

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  • Figure 3. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLS2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLS2 (1:2000).

    all(3)

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产品名称
Anti-GLS2 Antibody (Clone#OTI1C12)
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
GLS2; GA; Glutaminase liver isoform; mitochondrial; GLS; EC 3.5.1.2; L-glutaminase; L-glutamine amidohydrolase
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, IHC
基因名称
GLS2
克隆号
OTI1C12
宿主
Mouse
抗体亚型
IgG2a
免疫原
Full length human recombiant protein of human GLS2 (NP_037399) produced in E.coli.
计算分子量
66 kDa
实际分子量
66 kDa
成分
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]
Uniprot ID
Q9UI32  
文献引用格式
GLS2 Antibody (Clone#OTI1C12) (Boster Biological Technology, Wuhan, China. Catalog#M05334-1)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
基因名全称
glutaminase 2
蛋白名全称
Glutaminase liver isoform, mitochondrial
推荐稀释比
Western blot (WB):1:2000
Immunohistochemistry (IHC):1:2000

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    Anti-GLS2 Antibody (Clone#OTI1C12)

    筛选器: All IHC WB

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