HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLDN6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLDN6.
Anti-CLDN6 mouse monoclonal antibody (M08182-2) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CLDN6.