产品名称
Anti-Hu CD57/B3GAT1 Antibody (Clone#TB01)
指标别称
B3GAT1; CD57; GlcAT P; GLCATP; GlcUAT P; Glucuronosyltransferase P; HNK 1; HNK1; LEU7; NK 1
应用范围
FCM, WB, IHC, IHC-F, ICC
免疫原
A pool of neuroblastoma cell lines. The mouse monoclonal antibody TB01 recognizes CD57, a carbohydrate extracellular antigen present mainly on NK cells, NK T cells, and in neural tissue.
成分
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
纯化方式
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
保存条件
Store at 2-8°C. Do not freeze.
背景资料
CD57, also known as HNK1 or Leu7, is a sulphated trisaccharide (3-O-sulfoglucuronic acid beta1-3 Gal beta1-4 GlcNAc) attached to several glycoproteins, including CD56, myelin glycoprotein PO, and neural cell adhesion molecule L1, as well as on glycolipids and chondroitin sulphate proteoglycans in the nervous system. It serves as a NK cell marker and it is expressed on well differentiated prostate cancers and uveal and cutaneous melanoma. CD57+ T cells are implicated as suppressors of T-cell responses.
文献引用格式
Hu CD57/B3GAT1 Antibody (Clone#TB01) (Boster Biological Technology, Wuhan, China. Catalog#M09548-1)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC-免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
Beta-1,3-glucuronyltransferase 1
蛋白名全称
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
推荐稀释比
Flow cytometry: 1-4 µg/ml.
Immunohistochemistry: 5-10 µg/ml.