产品名称
Anti-Ms Ly6G Purified Low Endotoxin Antibody (Clone#RB6-8C5)
应用范围
FCM, IP, WB, IHC, FunctionalAssay
免疫原
Murine granulocytes. The rat monoclonal antibody RB6-8C5 detects Ly6G component of Gr-1 antigen, a commonly used surface marker of neutrophils.
成分
Phosphate buffered saline (PBS), pH 7.4
纯化方式
Purified by protein-A affinity chromatography.
保存条件
Store at 2-8°C. Do not freeze.
背景资料
Ly6G is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. Ly6G is a good marker for detection of peripheral neutrophils. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. Physiological role of Ly6G remains still unclear. Its treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses.
文献引用格式
Ms Ly6G Purified Low Endotoxin Antibody (Clone#RB6-8C5) (Boster Biological Technology, Wuhan, China. Catalog#M30404)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC-免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
Lymphocyte antigen 6 complex, locus G
推荐稀释比
Functional application: Neutrophil depletion.
Immunoprecipitation: 1-2 µg / 100-500 µg of protein.
Western blotting: 1 µg/ml.
Flow cytometry: 1-4 µg/ml