产品名称
Anti-GST Tag Antibody (Clone#5A7)
规格/价格
50μl/1180 100μl/1960
免疫原
This antibody is produced by immunizing animals with a synthetic GST tag peptide corresponding to KLH.
成分
500ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA and 50% glycerol.
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
A GST-Tag is often used to separate and purify proteins that contain the GST-fusion protein. The Tag is 220 amino acids (roughly 26KDa), in size is quite large. It is fused to the N-terminus of a protein. However, many commercially available sources of GST-Tagged plasmids include a thrombin domain for cleavage of the GST Tag during protein purification.
文献引用格式
GST Tag Antibody (Clone#5A7) (Boster Biological Technology, Wuhan, China. Catalog#M30973)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC-免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐稀释比
Western blot (WB): | 1:500-5000 |