产品名称
Anti-His Tag Antibody
规格/价格
50μl/1180 100μl/1960 150μl/2780
免疫原
This antibody is produced by immunizing animals with a synthetic His tag peptide corresponding to KLH.
成分
1 mg/ml antibody with PBS ,0.02% NaN3 , 1mg BSA and 50% glycerol.
保存条件
12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
A Polyhistidine-Tag is an amino acids motif in proteins that consists of at least six histidine(His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-Tag, 6xHis-Tag, His6 Tag and by the trademarked name His-Tag. Polyhistidine-Tags are often used for affinity purification of genetically modified proteins.
文献引用格式
His Tag Antibody (Boster Biological Technology, Wuhan, China. Catalog#M30975-1)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
推荐稀释比
Western blot (WB): | 1:500-2000 |