| Western blot (WB): | 1:2000 | 
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:100 | 

Flow cytometric Analysis of permeabilized Hela cells, using anti-TGFB1 antibody, (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:100).

Immunofluorescent staining of HeLa cells using anti-TGFB1 mouse monoclonal antibody (1:100).

Anti-TGFB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TGFB1 (1:100).

Flow cytometric Analysis of permeabilized HCT116 cells, using anti-TGFB1 antibody, (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:100).

Flow cytometric Analysis of permeabilized SF295 cells, using anti-TGFB1 antibody, (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:100).

Western blot analysis of extracts (35ug) from 5 different cell lines by using anti-TGFB1 monoclonal antibody (1:500).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TGFB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TGFB1 (Cat# MA00019)(1:2000).

Flow cytometric Analysis of permeabilized Hela cells, using anti-TGFB1 antibody, (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:100).

Immunofluorescent staining of HeLa cells using anti-TGFB1 mouse monoclonal antibody (1:100).

Anti-TGFB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TGFB1 (1:100).

Flow cytometric Analysis of permeabilized HCT116 cells, using anti-TGFB1 antibody, (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:100).

Flow cytometric Analysis of permeabilized SF295 cells, using anti-TGFB1 antibody, (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:100).

Western blot analysis of extracts (35ug) from 5 different cell lines by using anti-TGFB1 monoclonal antibody (1:500).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TGFB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TGFB1 (Cat# MA00019)(1:2000).






