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Anti-IL-6 Antibody (Clone#OTI3G9)

Mouse monoclonal antibody

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MA00102

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 5-7天
  • Figure 1. Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00102)

    all(19)
  • Figure 2. Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00102)

    all(19)
  • Figure 3. Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00102)

    all(19)
  • Figure 4. Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00102)

    all(19)
  • Figure 5. Western blot analysis of recombinant IL6 protein (0.02ug) by using anti-IL6 antibody (Cat# MA00102).

    all(19)
  • Figure 6. Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00102)

    all(19)
  • Figure 7. Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00102)

    all(19)
  • Figure 8. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00102)

    all(19)
  • Figure 9. Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00102)

    all(19)
  • Figure 10. Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00102)

    all(19)
  • Figure 11. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00102)

    all(19)
  • Figure 12. Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00102)

    all(19)
  • Figure 13. Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00102)

    all(19)
  • Figure 14. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00102)

    all(19)
  • Figure 15. Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00102)

    all(19)
  • Figure 16. Anti-IL-6 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IL-6 .

    all(19)
  • Figure 17. Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00102)

    all(19)
  • Figure 18. Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00102)

    all(19)
  • Figure 19. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-6 (Cat# MA00102).

    all(19)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称
Anti-IL-6 Antibody (Clone#OTI3G9)
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; HSF; Hybridoma growth factor; IL 6; IL6; IL-6; Interferon beta 2; Interleukin 6
产品类型
Monoclonal
检验物种
human
应用范围
WB, IHC, IF
基因名称
IL6
克隆号
OTI3G9
宿主
Mouse
抗体亚型
IgG2a
免疫原
Human recombinant protein fragment corresponding to amino acids 29-212 of human IL-6 (NP_000591) produced in E.coli.
计算分子量
24 kDa
实际分子量
23.5 kDa
成分
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
Interleukin-6(IL-6) is a protein that in humans is encoded by the IL6 gene. IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. Bowcock et al.(1988) assigned the IL6 gene to chromosome 7p21. By in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, Sutherland et al.(1988) mapped the IL-6 gene to chromosome 7p15.
Uniprot ID
P05231  
文献引用格式
IL-6 Antibody (Clone#OTI3G9) (Boster Biological Technology, Wuhan, China. Catalog#MA00102)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
基因名全称
interleukin 6
蛋白名全称
Interleukin-6
推荐稀释比
Western blot (WB):1:1000
Immunohistochemistry (IHC):1:50
Immunofluorescence (IF):1:100

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