Western blot (WB): | 1:400~2000 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:100 |
Figure 1. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NFKBIA monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Figure 3. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NFKBIA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NFKBIA.
Figure 2. Immunofluorescent staining of HeLa cells using anti-NFKBIA mouse monoclonal antibody.
Figure 1. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NFKBIA monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Figure 3. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NFKBIA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NFKBIA.
Figure 2. Immunofluorescent staining of HeLa cells using anti-NFKBIA mouse monoclonal antibody.