Multiplex ELISA Kit For Human Cytokine - Screen (16-plex)

96T/32000

IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-15, IL-17A, IL-23, TNFα, TNFβ

human

Serum, EDTA/Heparin Plasma

Min 25 µl

2.25 hours

All analytes < 1% intra-panel cross-reactivity

Planar Based Multiplexed ELISA

96-well solid plate

The Multiplex Human Cytokine – Screen (16-plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. Multiple analyte measurements are achieved by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well of a 96-well plate. Our high-quality reagents help ensure the accuracy and precision of your results.
Using only 25 µl of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 2.25 hours. The Multiplex Human Cytokine – Screen (16-plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample.

Analyte	Assay Type	Calibrator Range	Upper Limit of Quantification (ULOQ)	Lower Limit of Quantification (LLOQ)	Limit of Detection
IFNγ	Sandwich	8000 -10.97 (pg/mL)	5600 (pg/mL)	14.3 (pg/mL)	4.95 (pg/mL)
IL-1α	Sandwich	4000 -5.49 (pg/mL)	2800 (pg/mL)	7.1 (pg/mL)	5.43 (pg/mL)
IL-1β	Sandwich	12226 -16.77 (pg/mL)	8558 (pg/mL)	21.8 (pg/mL)	12.40 (pg/mL)
IL-2	Sandwich	5213 – 7.15 (pg/mL)	3649 (pg/mL)	9.3 (pg/mL)	4.12 (pg/mL)
IL-4	Sandwich	1676 – 2.30 (pg/mL)	1173 (pg/mL)	3.0 (pg/mL)	1.79 (pg/mL)
IL-5	Sandwich	3000 – 4.12 (pg/mL)	2100 (pg/mL)	5.3 (pg/mL)	2.90 (pg/mL)
IL-6	Sandwich	3000 – 4.12 (pg/mL)	2100 (pg/mL)	5.3 (pg/mL)	3.47 (pg/mL)
IL-8	Sandwich	2000 – 2.74 (pg/mL)	1400 (pg/mL)	3.6 (pg/mL)	1.37 (pg/mL)
IL-10	Sandwich	8039 – 11.03 (pg/mL)	5627 (pg/mL)	14.3 (pg/mL)	10.99 (pg/mL)
IL-12p70	Sandwich	3000 – 4.12 (pg/mL)	2100 (pg/mL)	5.3 (pg/mL)	3.20 (pg/mL)
IL-13	Sandwich	3000 – 4.12 (pg/mL)	2100 (pg/mL)	5.3 (pg/mL)	4.12 (pg/mL)
IL-15	Sandwich	10733 – 14.72 (pg/mL)	7513 (pg/mL)	19.1 (pg/mL)	6.87 (pg/mL)
IL-17A	Sandwich	16772 – 23.01 (pg/mL)	11740 (pg/mL)	29.9 (pg/mL)	22.57 (pg/mL)
IL-23	Sandwich	41700 – 57.20 (pg/mL)	21000 (pg/mL)	120 (pg/mL)	48.65 (pg/mL)
TNFα	Sandwich	6500 – 8.92 (pg/mL)	4522 (pg/mL)	11.5 (pg/mL)	7.74 (pg/mL)
TNFβ	Sandwich	4000 – 5.49 (pg/mL)	2800 (pg/mL)	7.1 (pg/mL)	5.29 (pg/mL)

Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization.
Reagents Include:
Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (3): Adhesive strips

Multiplex ELISA Kit For Human Cytokine - Screen (16-plex) (Boster Biological Technology, Wuhan, China. Catalog#MEK1005)