Multiplex ELISA Kit For Human Cytokine - Inflammation (9-plex)

96T/24000

IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, TNFα

human

Serum, EDTA/Heparin Plasma

Min 25 µl

2.25 hours

All analytes < 1% intra-panel cross-reactivity

Planar Based Multiplexed ELISA

96-well solid plate

The Multiplex Human Cytokine HS (9-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 9 biomarkers or analytes. Using only 25 µl of sample per well, up to 80 samples can be assayed for all 9 markers in the panel within 2.25 hours.
The Multiplex Human Cytokine HS (9-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 9 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Analyte	Assay Type	Calibrator Range	Upper Limit of Quantification (ULOQ)	Lower Limit of Quantification (LLOQ)	Limit of Detection
IFNγ	Sandwich	8000 -10.97 (pg/mL)	5600 (pg/mL)	14.3 (pg/mL)	4.95 (pg/mL)
IL-1α	Sandwich	4000 -5.49 (pg/mL)	2800 (pg/mL)	7.1 (pg/mL)	5.43 (pg/mL)
IL-1β	Sandwich	12226 -16.77 (pg/mL)	8558 (pg/mL)	21.8 (pg/mL)	12.40 (pg/mL)
IL-2	Sandwich	5213 – 7.15 (pg/mL)	3649 (pg/mL)	9.3 (pg/mL)	4.12 (pg/mL)
IL-4	Sandwich	1676 – 2.30 (pg/mL)	1173 (pg/mL)	3.0 (pg/mL)	1.79 (pg/mL)
IL-6	Sandwich	3000 – 4.12 (pg/mL)	2100 (pg/mL)	5.3 (pg/mL)	3.47 (pg/mL)
IL-8	Sandwich	2000 – 2.74 (pg/mL)	1400 (pg/mL)	3.6 (pg/mL)	1.37 (pg/mL)
IL-10	Sandwich	8039 – 11.03 (pg/mL)	5627 (pg/mL)	14.3 (pg/mL)	10.99 (pg/mL)
TNFα	Sandwich	6500 – 8.92 (pg/mL)	4522 (pg/mL)	11.5 (pg/mL)	7.74 (pg/mL)

Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.
Reagents Include:
Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (3): Adhesive strips

Multiplex ELISA Kit For Human Cytokine - Inflammation (9-plex) (Boster Biological Technology, Wuhan, China. Catalog#MEK1008)