产品名称
Multiplex ELISA Kit For Human Cytokine Panel 1 (6-Plex)
检测蛋白
IFNγ, IL-1α, IL-1β, IL-6, IL-10, TNFα
样本类型
Serum, EDTA/Heparin Plasma
分析物交叉反应
All analytes < 1% intra-panel cross-reactivity
实验类型
Planar Based Multiplexed ELISA
产品描述
The Multiplex Human Cytokine Panel 1 (6-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of six biomarkers or analytes (IL-1β, IL-6, TNFα, IFN-γ, IL-1α, IL-10).
Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Multiplex plates are built by absorbing six distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.
Using only 25 µl of sample per well, up to 80 samples can be assayed for all six markers in the panel in 2.25 hours. Multiplex Arrays provide scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.
检测范围
Analyte | Assay Type | Calibrator Range | Upper Limit of Quantification (ULOQ) | Lower Limit of Quantification (LLOQ) | Limit of Detection |
---|
IFNγ | Sandwich | 8000 -10.97 (pg/mL) | 5600 (pg/mL) | 14.3 (pg/mL) | 4.95 (pg/mL) |
IL-1α | Sandwich | 4000 -5.49 (pg/mL) | 2800 (pg/mL) | 7.1 (pg/mL) | 5.43 (pg/mL) |
IL-1β | Sandwich | 12226 -16.77 (pg/mL) | 8558 (pg/mL) | 21.8 (pg/mL) | 12.40 (pg/mL) |
IL-6 | Sandwich | 3000 – 4.12 (pg/mL) | 2100 (pg/mL) | 5.3 (pg/mL) | 3.47 (pg/mL) |
IL-10 | Sandwich | 8039 – 11.03 (pg/mL) | 5627 (pg/mL) | 14.3 (pg/mL) | 10.99 (pg/mL) |
TNFα | Sandwich | 6500 – 8.92 (pg/mL) | 4522 (pg/mL) | 11.5 (pg/mL) | 7.74 (pg/mL) |
试剂盒内容
Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.
Reagents Include:
Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (3): Adhesive strips
文献引用格式
Multiplex ELISA Kit For Human Cytokine Panel 1 (6-Plex) (Boster Biological Technology, Wuhan, China. Catalog#MEK1010)